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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
Fecha : |
17/04/2024 |
Actualizado : |
17/04/2024 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Autor : |
AREVALO, A.P.; BASIKA, T.; ANCHETA, S.; PERELMUTER, K.; RICCIARDI, A.; BOLLATI-FOGOLÍN, M.; CRISPO, M. |
Afiliación : |
ANA PAULA AREVALO, Institut Pasteur de Montevideo, Laboratory Animals Biotechnology Unit, Montevideo, Uruguay; TATIANA BASIKA, Unidad Mixta entre Instituto Pasteur de Montevideo e INIA (UMPI), Montevideo, Uruguay; Institut Pasteur de Montevideo, Laboratory Animals Biotechnology Unit, Montevideo, Uruguay; Institut Pasteur de Montevideo, Cell Biology Unit, Montevideo, Uruguay; SERGIO ANCHETA, Institut Pasteur de Montevideo, Laboratory Animals Biotechnology Unit, Montevideo, Uruguay; KAREN PERELMUTER, Institut Pasteur de Montevideo, Cell Biology Unit, Montevideo, Uruguay; ALEJANDRO RICCIARDI; MARIELA BOLLATI-FOGOLÍN, Institut Pasteur de Montevideo, Cell Biology Unit, Montevideo, Uruguay; MARTINA CRISPO, Institut Pasteur de Montevideo, Laboratory Animals Biotechnology Unit, Montevideo, Uruguay. |
Título : |
3R's applied to in vivo biological activity of recombinant human erythropoietin assay. |
Complemento del título : |
Technical Article. |
Fecha de publicación : |
2023 |
Fuente / Imprenta : |
Biological Models Research and Technology Journal. 2023, Volume 3, Issue 1, e00012023. http://dx.doi.org/10.4322/2675-9225.00012023 -- OPEN ACCESS. |
ISSN : |
2675-9225 |
DOI : |
10.4322/2675-9225.00012023 |
Idioma : |
Inglés |
Notas : |
Article history: Submitted date 9 February 2023, Accepted date 19 April 2023. -- Correspondence: Crispo, M.; Institut Pasteur de Montevideo, Laboratory Animals Biotechnology Unit, Montevideo, Uruguay; email:crispo@pasteur.edu.uy -- FUNDING: This work was supported by FOCEM (MERCOSUR Structural Convergence Fund), COF 03/11 and ANII (EQL_2013_X_1_2). -- Document type: Article Bronze Open Access. --
Publisher: Brazilian Society for Laboratory Animal Science. |
Contenido : |
ABSTRACT.- An enhanced protocol for the evaluation of in vivo biological activity of recombinant human erythropoietin (rhEPO) assay in mice is described, following European Pharmacopoeia (Ph. Eur.) version 10.0 guideline and optimized by applying the principles of Replacement, Reduction and Refinement (3Rs) in animal experimentation. In Laboratory Animal Science, one of the main principles is to optimize the experimental protocols in order to achieve the best results with the lowest number of animals and refine the procedures to avoid unnecessary suffering. The main objective of this protocol is to comply with international guidelines for rhEPO evaluation, applying refinement on procedures and reduction in animal use. Some of the features included in this protocol are the increase in number of rhEPO batches tested simultaneously against an international standard, leading to a substantial reduction in the number of animal's used, and refinement on animal handling techniques for subcutaneous drug administration and blood withdrawal. The implemented improvements were validated by reticulocyte estimation to ensure compliance with international criteria established for this trial and institutional quality management system. © 2023, Brazilian Society for Laboratory Animal Science. All rights reserved. |
Palabras claves : |
3R’s; Biological activity; Erythropoietin; European Pharmacopeia; Mice; UNIDAD MIXTA PASTEUR + INIA. |
Asunto categoría : |
A50 Investigación agraria |
URL : |
https://bmrtsbcaljournal.com/article/10.4322/2675-9225.00012023/pdf/bmrt-3-1-e00012023.pdf
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Marc : |
LEADER 02721naa a2200301 a 4500 001 1064596 005 2024-04-17 008 2023 bl uuuu u00u1 u #d 022 $a2675-9225 024 7 $a10.4322/2675-9225.00012023$2DOI 100 1 $aAREVALO, A.P. 245 $a3R's applied to in vivo biological activity of recombinant human erythropoietin assay.$h[electronic resource] 260 $c2023 500 $aArticle history: Submitted date 9 February 2023, Accepted date 19 April 2023. -- Correspondence: Crispo, M.; Institut Pasteur de Montevideo, Laboratory Animals Biotechnology Unit, Montevideo, Uruguay; email:crispo@pasteur.edu.uy -- FUNDING: This work was supported by FOCEM (MERCOSUR Structural Convergence Fund), COF 03/11 and ANII (EQL_2013_X_1_2). -- Document type: Article Bronze Open Access. -- Publisher: Brazilian Society for Laboratory Animal Science. 520 $aABSTRACT.- An enhanced protocol for the evaluation of in vivo biological activity of recombinant human erythropoietin (rhEPO) assay in mice is described, following European Pharmacopoeia (Ph. Eur.) version 10.0 guideline and optimized by applying the principles of Replacement, Reduction and Refinement (3Rs) in animal experimentation. In Laboratory Animal Science, one of the main principles is to optimize the experimental protocols in order to achieve the best results with the lowest number of animals and refine the procedures to avoid unnecessary suffering. The main objective of this protocol is to comply with international guidelines for rhEPO evaluation, applying refinement on procedures and reduction in animal use. Some of the features included in this protocol are the increase in number of rhEPO batches tested simultaneously against an international standard, leading to a substantial reduction in the number of animal's used, and refinement on animal handling techniques for subcutaneous drug administration and blood withdrawal. The implemented improvements were validated by reticulocyte estimation to ensure compliance with international criteria established for this trial and institutional quality management system. © 2023, Brazilian Society for Laboratory Animal Science. All rights reserved. 653 $a3R’s 653 $aBiological activity 653 $aErythropoietin 653 $aEuropean Pharmacopeia 653 $aMice 653 $aUNIDAD MIXTA PASTEUR + INIA 700 1 $aBASIKA, T. 700 1 $aANCHETA, S. 700 1 $aPERELMUTER, K. 700 1 $aRICCIARDI, A. 700 1 $aBOLLATI-FOGOLÍN, M. 700 1 $aCRISPO, M. 773 $tBiological Models Research and Technology Journal. 2023, Volume 3, Issue 1, e00012023. http://dx.doi.org/10.4322/2675-9225.00012023 -- OPEN ACCESS.
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INIA Las Brujas (LB) |
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Registros recuperados : 6 | |
1. | | CARVALHO, F. K. L.; NASCIMENTO, E. M.; ROCHA, B. P.; MENDONÇA. F. S.; VESCHI, J. L. A.; SILVA, S. M. M. S.; MEDEIROS, R. M. T.; RIET-CORREA, F. Hybanthus calceolaria poisoning in cattle. Journal of Veterinary Diagnostic Investigation, v. 26, p. 1-4, aug. 2014.Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : A - 2 |
Biblioteca(s): INIA La Estanzuela. |
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2. | | NASCIMENTO, E.M.; CAMPOS, É.M.; MAIA, L.Â.; MEDEIROS, R.M.T.; ALCÂNTARA, M.D.B.; VILAR, S.D.; RIET-CORREA, F. Megaesophagus in sheep and goats. [Megaesôfago em ovinos e caprinos]. Ciencia Rural, v. 46 ,n.8,p. 1450-1455. 2016. Article history: Received 10.10.15 //Approved 02.08.16// Returned by the author 03.28.16.Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
Biblioteca(s): INIA La Estanzuela. |
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4. | | CAMPOS, E.M.; MAIA, L.A.; OLINDA, R.G.; NASCIMENTO, E.M.; MELO, D.B.; DANTAS, A.F.M.; RIET-CORREA, F. Poisoning by Senna obtusifolia in sheep. Pesquisa Veterinaria Brasileira 38(8):1471-1474, agosto 2018. Article History: Received on July 11, 2017.//Accepted for publication on July 26, 2017.Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
Biblioteca(s): INIA La Estanzuela. |
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6. | | SLVEIRA, A. M.; NASCIMENTO, E. M.; KONRADT, G.; NETO, E. G. M.; DRIEMEIER, D.; GALIZA, J. N. G.; DANTAS, A. F. M.; RIET-CORREA, F. Tuberculosis of the central nervous system in cattle in Paraíba, Brazil. [Tuberculose no sistema nervoso central de bovinos na Paraíba.] Pesquisa Veterinaria Brasileira 38(11):2099-2108, 1 November 2018. OPEN ACCESS Article history: Received on June 26, 2018. // Accepted for publication on July 12, 2018.Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
Biblioteca(s): INIA La Estanzuela. |
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Registros recuperados : 6 | |
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